The facility would serve as a "Singe-window service system" providing naotoxicological research based solutions to stakeholders encompassing government and academic labs and industries. The services to be soon provided by the facility include:

Quality control of nano-agri products (Nano-characterization and assessment of safety to human and environment)

Nanoproducts Norms (National) Norms (International)
Specifying nano objects ISO/TS 12805:2011 -
Nanoparticle in powder form-Characteristics and measurement ISO/TS 17200:2003 -
Electrolyte Conductivity - NIST-NCL PCC-12
Measurement of Particle size and shape distributions by  SEM ISO/DIS 19749 NIST-NCL PCC-15
Measurement of Particle size and shape distributions by TEM ISO 21363:2020 NIST-NCL PCC-7
Evaluation of mean size of nano-objects in liquid dispersions by static multiple light scattering (SMLS) ISO/CD TS 21357 NIST-NCL PCC-1
Zeta potential - NIST-NCL PCC-2
Particle concentration and Size - NIST-NCL PCC-20
Qualtification of Surface coating on metallic nanoparticles using Thermo gravimetric analysis - NIST-NCL PCC-17

In vitro nano-safety assessments using mammalian cell lines and NCL/OECD guidelines

Cytotoxicity assays

  • Neutral red uptake
  • LDH release
  • MTT
  • Trypan Blue

(paralleled by cell morphology analyses by microscopy for cross checking)


  • Comet assay
  • DNA double-strands break assay

Ex vivo nanosafety assessment using human blood and the standard method from NIST-NCL method and ISO-10993-4 guideline

  • Interference of nanoparticles with coagulation cascade and interaction with blood components.

In vivo nano-safety assessment using environmental species

  • Bacteria (Agar well diffusion method ISO 20776-1:2006)
  • C. elegans (Using GCeCP methods)
  • Earthworm (OECD TG 222)
  • fresh water and marine micro-algae (OECD TG 201)
  • Daphnia (OECD TG 202)
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