Page 87 - Abstract Book - ISNPMP 2016
P. 87
Kanika Chowdhary P 14
BIODIVERSITY AND ANTIFUNGAL ACTIVITY OF
ENDOPHYTIC FUNGI ISOLATED FROM AN INDIAN
MEDICINAL PLANT CHLOROPHYTUM BORIVILIANUM
SANT & FERN.
Kanika Chowdhary1and Nutan Kaushik2
1TERI University, 10 Institutional Area, Vasant Kunj, New Delhi-110070, India
2 The Energy and Resources Institute (TERI), India Habitat Center, Lodhi Road, New Delhi – 110003, India,
The endophytic fungal community associated with Chlorophytum borivilianum Sant & Fern., Indian medicinal
plant was investigated and their potential as biocontrol agent against phytopathogens Botrytis cinerea,
Fusarium oxysporum, Sclerotinia sclerotiorum and Rhizoctonia solani was explored. From 209 leaf tissues of C.
borivilianum brought from Delhi (different sampling time) and Hyderabad 51 endophytic fungal isolates were
attained. Sequence analysis of PCR amplified rDNA ITS region grouped these endophytic fungal isolates into
12 genera. Overall, colonization rate from different regions and collection time was found out to be 24.4%.
The most abundant species was Fusarium proliferatum (17.6%). Shannon diversity index (H’) was found to be
higher in Delhi (1.850) than Hyderabad (0.693) in 2010. Plant pathogens such as Rhizoctonia bataticola and
Setosphaeria rostrata have been reported to be harbored as endophytes inside C. borivilianum for the first
time. Bipolaris maydis, Diaporthe phaseolorum, Fusarium solani, Macrophomina phaseolina and Rhizoctonia
bataticola were recovered from Delhi during first collection as deduced by principal component analysis. 9.8%
of endophytic fungal isolates exhibited antiphytopathogenicity. Fusarium proliferatum recovered from Delhi
in 2011 displayed highly effective antifungal activity against all phytopathogens with IC50 value ranging from
0.527 to 2.119 mg/ml.
86 ISNPMP 2016
BIODIVERSITY AND ANTIFUNGAL ACTIVITY OF
ENDOPHYTIC FUNGI ISOLATED FROM AN INDIAN
MEDICINAL PLANT CHLOROPHYTUM BORIVILIANUM
SANT & FERN.
Kanika Chowdhary1and Nutan Kaushik2
1TERI University, 10 Institutional Area, Vasant Kunj, New Delhi-110070, India
2 The Energy and Resources Institute (TERI), India Habitat Center, Lodhi Road, New Delhi – 110003, India,
The endophytic fungal community associated with Chlorophytum borivilianum Sant & Fern., Indian medicinal
plant was investigated and their potential as biocontrol agent against phytopathogens Botrytis cinerea,
Fusarium oxysporum, Sclerotinia sclerotiorum and Rhizoctonia solani was explored. From 209 leaf tissues of C.
borivilianum brought from Delhi (different sampling time) and Hyderabad 51 endophytic fungal isolates were
attained. Sequence analysis of PCR amplified rDNA ITS region grouped these endophytic fungal isolates into
12 genera. Overall, colonization rate from different regions and collection time was found out to be 24.4%.
The most abundant species was Fusarium proliferatum (17.6%). Shannon diversity index (H’) was found to be
higher in Delhi (1.850) than Hyderabad (0.693) in 2010. Plant pathogens such as Rhizoctonia bataticola and
Setosphaeria rostrata have been reported to be harbored as endophytes inside C. borivilianum for the first
time. Bipolaris maydis, Diaporthe phaseolorum, Fusarium solani, Macrophomina phaseolina and Rhizoctonia
bataticola were recovered from Delhi during first collection as deduced by principal component analysis. 9.8%
of endophytic fungal isolates exhibited antiphytopathogenicity. Fusarium proliferatum recovered from Delhi
in 2011 displayed highly effective antifungal activity against all phytopathogens with IC50 value ranging from
0.527 to 2.119 mg/ml.
86 ISNPMP 2016
